the samples are stored in a specific fridge located in the DNA science area...
The samples that are listed on this sheet are the samples that have been added to the plate...
(In the fridge) the DNA extract is a liquid that's placed in a tube and the tube has specific labelling on it and these tubes are placed on a rack which is on a shelf...
When I've gone to pick up these samples, I've placed them (in a rack) in the order of the sample sheet...
Item 11.1 was extracted on 14 December 2007...
I'd go to the quantification rack based on what's on the sample sheet and position them into the rack according to what position I'd load them onto the plate...
First you set up the samples that require quantification in a rack in the order of where they will be loaded onto the plate...and then you would actually load those samples onto the...plate....
all transfer steps are witnessed by another analyst who is authorised ...to ensure that the correct samples are being loaded in the correct positions on the plate...
(The sample tubes) are always capped...
I'd then go and set up the 7500 plate. The setup is performed in an isolated area in its own separate hood. Prior to use of the hood it is decontaminated with hydrochloride...
I would set up the actual plate which is a sterile plastic plate which contains the 96 wells...
two microlitres (are) taken out of the tube and placed in the well....
only one tube is handled at any given time...I would open that tube. I would use the pipette which is the instrument used to measure volume and on top of the end of the pipette a tip is placed, which is a sterile tip and which is only used once...
I would take two microlitres from that tube. I would close that tube immediately and place it back into the rack and place the two microlitres of sample into the relevant position that it is required in...