The present case is concerned with their relation to the
commercial production of pressed yeast. The procedure followed
in its production falls naturally into two parts. The grain is mixed
with the malt and mashed at a proper temperature so that, to as
great an extent as possible, the starch will become soluble sugar or
dextrose. The mash must stand 'some time and much bacterial and
chemical action takes place. As yeast, a micro-organism, is to be
grown in it, the degree of acidity of the mash must be controlled.
At one time, when the mash was considered ready, it was sown
directly with yeast, which propagated in it. This was called the
"Vienna method." But for many years another practice has pre-
vailed. The mash is prepared from grain which is softened with
water and crushed, not ground. The nutrient materials, nitro-
genous or protein as well as sugars, are drawn off in liquor. The
mash is washed again and the liquid of the wash filtered off, and this
is done yet a third and a fourth time. These filtrations are the wort
into which the seed yeast is sown. It was established many years
ago, by Pasteur it is said, that in the absence of atmospheric oxygen
yeast consumed in its growth a quantity of sugar surprisingly greater
than in the presence of air. Accordingly the liquid wort is aerated
throughout the period of the yeast fermentation. The control of
temperature, sterilization, the use of inorganic contributions to the
nutriment and many other matters are involved in the process.
When the yeast has broken down the nutrient properties of the wort
to such an extent that the ferment should be terminated, the yeast
is separated mechanically from the liquor. The alcohol contained in
the liquor may be worth recovering and this is done by distillation.
The sugar content of the wort, determining, as it does, its density,
is ascertainable by a saccharometer. Tables have been constructed
by means of which the concentration of the wort can always be found.
The proportion of alcohol in the wort is also ascertainable without
difficulty. Alcohol is, of course, a product of the fermentation and
its increase in the wort appeared to be an unavoidable concomitant
of the growth of the yeast. But alcohol, at any rate in particular
conditions and proportions not exactly determined, proved a most
powerful retarding agent in the propagation of yeast. It was said
to bea poison for the yeast. Rich worts produced alcohol; " strong